Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: PCR primer sequences and NCBI accession numbers for mucin genes/transcripts. The letter P denotes sequences that are predicted.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques:

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: The peripheral cornea expresses MUC1, MUC4 and MUC16 at higher levels than the central cornea. Epithelium was debrided from both the peripheral (~75% corneal surface area) and central (~25% corneal surface area) cornea and quantitative PCR was performed. In all species examined (A-rhesus macaque, B-canine, C-rabbit), mucin expression tended to be higher in the peripheral cornea when compared with the central cornea. Data represents relative mucin expression normalized to the respective housekeeping gene per species (Table 1, n = 6 eyes per species from different individuals). Error bars represent standard error of the mean. Asterisk indicates p ≤ 0.05.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: Corneal mucin mRNA transcript expression pattern differs between species. Quantitative PCR of mucin mRNA transcript expression in humans (A), rhesus macaques (B), dogs (C) and rabbits (D). Similar patterns of mucin mRNA expression existed in humans, rhesus macaques and rabbits with MUC16 being expressed to the highest levels, followed by MUC1 and MUC4 expression being the lowest detected. The rabbit had a unique pattern of mucin mRNA expression with MUC4 being expressed at the highest level, MUC1 and MUC16 being expressed at lower levels. Data represents relative mucin expression normalized to the respective housekeeping gene per species (Table 1, n = 5 eyes per species from different individuals). Error bars represent standard error of the mean.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Experimental eye research

Article Title: Species variation and spatial differences in mucin expression from corneal epithelial cells

doi: 10.1016/j.exer.2016.09.001

Figure Lengend Snippet: MUC16 glycoprotein was detectable in human, rhesus macaque and canine corneal epithelial extracts. Using a human-specific MUC16 antibody (OC125), large high molecular weight protein was detected a human, rhesus macaque and canine corneal epithelial extracts, whereas no protein was detected in rabbit epithelial extracts.

Article Snippet: Human MUC16 , Hs01065189_m1 , , {"type":"entrez-nucleotide","attrs":{"text":"NM_024690","term_id":"83367076","term_text":"NM_024690"}} NM_024690.

Techniques: High Molecular Weight

Journal: Journal of Clinical Investigation

Article Title: CXCL12+ fibroblastic reticular cells in lymph nodes facilitate immune tolerance by regulating T cell–mediated alloimmunity

doi: 10.1172/jci182709

Figure Lengend Snippet: Figure 2. Deletion of LTβR+ signaling on FRCs abrogates transplant tolerance. (A) Comparison of heart allograft survival among WT C57BL/6J, Ccl19CreLtbrfl/fl, and Ccl19CreLtbrfl/fl recipients with FRC injections (n = 7–9 mice/group). Graft survival data were combined from 3 independent experiments. (B) Representative images and comparison of CD3-, CD11b-, and collagen 1–stained heart allografts from WT C57BL/6J and Ccl19CreLtbrfl/fl recipients (n = 4 mice/group). Scale bars: 200 μm. (C) Flow cytometry analysis of DLNs from WT C57BL/6J and Ccl19CreLtbrfl/fl recipients (n = 4 mice/group). (D) Representative images and comparison of Foxp3-stained DLNs (n = 4 mice/group). Scale bars: 200 μm. (E) Flow cytometry analysis of transferred TCR Tg cells in DLNs (n = 4 mice/group). (F) Comparison of heart allograft survival among WT C57BL/6J recipients treated with anti-LTβR agonist mAb alone (20 μg i.v. on day 0), anti-CD40L alone (40 μg i.v. on day 0), and both anti-LTβR agonist mAb and anti-CD40L of BALB/c hearts (n = 6 mice/group). Graft survival data were combined from 2 independent experiments. log-rank test for graft survival. Student’s t test for comparisons between 2 groups. Data are represented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: As the standard protocol in this study, anti-CD40L mAb (MR1, 125 μg i.v. on days 0 and 1, BioXCell, BE0017-1) or CTLA4-Ig (Abatacept, 500 μg i.p. on day 0 and 250 μg i.p. on days 2, 4, 6, 8, Bristol-Myers Squibb) was administered to recipient mice.

Techniques: Comparison, Staining, Flow Cytometry

Journal: Journal of Clinical Investigation

Article Title: CXCL12+ fibroblastic reticular cells in lymph nodes facilitate immune tolerance by regulating T cell–mediated alloimmunity

doi: 10.1172/jci182709

Figure Lengend Snippet: Figure 3. CXCL12hi FRC subsets enriched for genes for T cell migration. (A) Unsupervised clustering of FRC subset clusters visualized with UMAP in LNSCs from naive LNs. (B) Violin plots of Ltbr and Cxcl12 expression among FRC subset clusters in naive LNs. (C) Violin plots of Ltbr and Cxcl12 expres- sion in total FRCs from naive LNs. (D) UMAP of SC populations in naive LNs and DLNs, highlighting Cxcl12 expression on MedRC, TRC, and PRC subsets. (E) Violin plots of Cxcl12 expression in FRC subsets from naive LNs and DLNs. (F) Volcano plot comparing CXCL12hi and CXCL12lo FRCs in mouse LNSCs from heart transplanted recipients with anti-CD40L treatment. (G) Top 20 overrepresented ontology pathways based on DEGs in mouse LNSCs. (H) Volcano plot comparing CXCL12hi and CXCL12lo FRCs in human LNSCs. (I) Top 20 overrepresented ontology pathways in human LNSCs. Common genes shared between mouse and human are highlighted with bold letters surrounded by squares (F and H). Ontology pathways related to chemotaxis and migration are highlighted with bold letters surrounded by squares (G and I). HTx, heart transplantation.

Article Snippet: As the standard protocol in this study, anti-CD40L mAb (MR1, 125 μg i.v. on days 0 and 1, BioXCell, BE0017-1) or CTLA4-Ig (Abatacept, 500 μg i.p. on day 0 and 250 μg i.p. on days 2, 4, 6, 8, Bristol-Myers Squibb) was administered to recipient mice.

Techniques: Migration, Expressing, Chemotaxis Assay, Transplantation Assay

Journal: Journal of Clinical Investigation

Article Title: CXCL12+ fibroblastic reticular cells in lymph nodes facilitate immune tolerance by regulating T cell–mediated alloimmunity

doi: 10.1172/jci182709

Figure Lengend Snippet: Figure 4. Ablation of CXCL12 of FRCs abrogates anti-CD40L mediated transplant tolerance. (A) Flow cytometry analysis of T cell compartments in naive LNs (n = 3 mice/group). (B) Representative intravital images of CMFDA-labeled CD3+ T cells injected into naive LNs. Yellow and white arrows indicate the migration of transferred CMFDA+ CD3+ T cells from inside HEVs into the parenchyma. Representative trajectories of transferred CD3+ T cells (gray, cyan, and magenta lines) and quantitative analysis of cell migration from inside HEVs toward the parenchyma for 20 minutes (n = 3). Scale bars: 100 μm. (C) In vivo T cell migration assay in a skin transplantation model. Representative images and quantitative analysis of CMFDA-labeled CD3+ T cells in DLNs (n = 5). Scale bars: 100 μm. (D) Comparison of heart allograft survival among the recipients under CTLA4 Ig treatment (n = 7 mice/group). Graft survival data were combined from 2 independent experiments. (E) Representative images and comparison of CD3-, CD11c-, and collagen 1–stained heart allografts (n = 5 mice/group). Scale bars: 200 μm. (F) Flow cytometry analysis of DLNs (n = 5 mice/group). log-rank test for graft survival. Student’s t test for comparisons between 2 groups. Data are represented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: As the standard protocol in this study, anti-CD40L mAb (MR1, 125 μg i.v. on days 0 and 1, BioXCell, BE0017-1) or CTLA4-Ig (Abatacept, 500 μg i.p. on day 0 and 250 μg i.p. on days 2, 4, 6, 8, Bristol-Myers Squibb) was administered to recipient mice.

Techniques: Flow Cytometry, Labeling, Injection, Migration, In Vivo, Cell Migration Assay, Transplantation Assay, Comparison, Staining

Journal: RSC Advances

Article Title: An electrochemically synthesized molecularly imprinted polymer for highly selective detection of breast cancer biomarker CA 15-3: a promising point-of-care biosensor †

doi: 10.1039/d4ra02051k

Figure Lengend Snippet: Selectivity studies using a competitive method between the target molecule and the interfering species. The interfering species studied were CEA, CA 125, and urea.

Article Snippet: Potassium hexacyano trihydrate (K 4 Fe(CN) 6 ·3H 2 O), potassium hexacyano dihydrate (K 3 Fe(CN) 6 ), sodium phosphate dibasic dihydrate (Na 2 HPO 4 ·2H 2 O) and sodium dihydrogen phosphate dihydrate (NaH 2 PO 4 ·2H 2 O) were purchased from Riedel de Haën; fetal bovine serum (FBS) was purchased from Alfa Aesar; sulphuric acid (H 2 SO 4 ) was obtained from BDH; urea was from Fagron; proteinase K and (3-acrylamidopropyl)trimethylammonium chloride (solution 75 wt% in H 2 O) (AMPTMA) was acquired from Sigma-Aldrich; carcinoembryonic antigen (CEA) was obtained from EastCostBio; Cancer Antigen 125 (CA-125) was from Hytest; CA 15-3 from host human (reference MBS536585) was purchased from MyBioSource.

Techniques: